Document Type
Article
Publication Date
6-4-2013
Department 1
Biology
Abstract
The salamander is the only tetrapod that regenerates complex body structures throughout life. Deciphering the underlying molecular processes of regeneration is fundamental for regenerative medicine and developmental biology, but the model organism had limited tools for molecular analysis. We describe a comprehensive set of germline transgenic strains in the laboratory-bred salamander Ambystoma mexicanum(axolotl) that open up the cellular and molecular genetic dissection of regeneration. We demonstrate tissue-dependent control of gene expression in nerve, Schwann cells, oligodendrocytes, muscle, epidermis, and cartilage. Furthermore, we demonstrate the use of tamoxifen-induced Cre/loxP-mediated recombination to indelibly mark different cell types. Finally, we inducibly overexpress the cell-cycle inhibitor p16INK4a, which negatively regulates spinal cord regeneration. These tissue-specific germline axolotl lines and tightly inducible Cre drivers and LoxP reporter lines render this classical regeneration model molecularly accessible.
Copyright Note
This is the publisher's version of the work. This publication appears in Gettysburg College's institutional repository by permission of the copyright owner for personal use, not for redistribution.
DOI
10.1016/j.stemcr.2013.03.002
Recommended Citation
Khattak, Shahryar, Maritta Schuez, Tobias Richter, Dunja Knapp, Saori L. Haigo, Tatiana Sandoval-Guzmán, Kristyna Hradlikova, Annett Duemmler, Ryan Kerney and Elly M. Tanaka. “Germline Transgenic Methods for Tracking Cells and Testing Gene Function During Regeneration in the Axolotl.” Stem Cell Reports 1.1 (2013): 90-103. Web.
Required Publisher's Statement
Original version is available from the publisher at: http://download.cell.com/stem-cell-reports/pdf/PIIS2213671113000039.pdf?intermediate=true
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