Mono- and Trivalent Ions around DNA: A Small-Angle Scattering Study of Competition and Interactions
The presence of small numbers of multivalent ions in DNA-containing solutions results in strong attractive forces between DNA strands. Despite the biological importance of this interaction, e.g., DNA condensation, its physical origin remains elusive.Wecarried out a series of experiments to probe interactions between short DNA strands as small numbers of trivalent ions are included in a solution containing DNA and monovalent ions. Using resonant (anomalous) and nonresonant small angle x-ray scattering, we coordinated measurements of the number and distribution of each ion species around the DNA with the onset of attractive forces between DNA strands. DNA-DNA interactions occur as the number of trivalent ions increases. Surprisingly good agreement is found between data and size-corrected numerical Poisson-Boltzmann predictions of ion competition for non- and weakly interacting DNAs. We also obtained an estimate for the minimum number of trivalent ions needed to initiate DNA-DNA attraction.
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Kurt, A., Xiangyun, Q., Suzette A., P., Jessica S., L., Hye Yoon, P., Lisa W., K., & Lois, P. (2008). Mono- and Trivalent Ions around DNA: A Small-Angle Scattering Study of Competition and Interactions. Biophysical Journal, 95, 287-295. http://dx.doi.org/10.1529/biophysj.107.123174
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